Table 1.
Biosensor | RBS sequencea | Amino acid mutations | Ligand | K1/2 (μM)b | Dynamic rangec | nd |
---|---|---|---|---|---|---|
Wild-type | AGAAGGT | - | ErA | 1.97 ± 0.03 | 65,000 | 3.8 ± 0.3 |
QCMS-3D6 | AGAAGGT | T17R | ErA | 0.92 ± 0.03 | 67,000 | 3.5 ± 0.2 |
QCMS-3F8 | AGAAGGT | T17A/M59S | ErA | 1.43 ± 0.03 | 64,000 | 2.5 ± 0.1 |
QCMS-5B4 | AGAAGGT | Q65M | ErA | 1.45 ± 0.03 | 63,000 | 2.6 ± 0.1 |
QCMS-5D7 | AGAAGGT | T17A/M59E | ErA | 1.11 ± 0.02 | 60,000 | 2.4 ± 0.1 |
EP-A3 | GGAAGGT | G76C | ErA | 0.39 ± 0.02 | 64,000 | 2.0 ± 0.1 |
EP-E7 | AGATGGT | V90I | ErA | 0.58 ± 0.02 | 70,000 | 2.5 ± 0.1 |
EP-H4 | AGAAGGC | R24H/K35N | ErA | 0.74 ± 0.03 | 68,000 | 2.4 ± 0.1 |
AA-A3 | AGAAGGT | G76C | ErA | 1.94 ± 0.02 | 61,000 | 3.4 ± 0.1 |
AA-E7 | AGAAGGT | V90I | ErA | 2.17 ± 0.3 | 63,800 | 3.1 ± 0.1 |
AA-H4 | AGAAGGT | R24H/K35N | ErA | 1.88 ± 0.03 | 63,700 | 3.6 ± 0.3 |
RBS-A3 | GGAAGGT | - | ErA | 0.52 ± 0.02 | 78,000 | 1.9 ± 0.1 |
RBS-E7 | AGATGGT | - | ErA | 0.64 ± 0.02 | 74,000 | 2.0 ± 0.1 |
RBS-H4 | AGAAGGC | - | ErA | 1.00 ± 0.02 | 75,000 | 2.2 ± 0.1 |
smRBS-1A1 | TTCAGGT | - | ErA | 0.19 ± 0.02 | 70,000 | 1.7 ± 0.3 |
smRBS-1G6 | CTGAGGT | - | ErA | 0.91 ± 0.04 | 64,000 | 5.5 ± 1.2 |
smRBS-2E1 | AAAGGTT | - | ErA | 1.44 ± 0.08 | 64,000 | 3.9 ± 0.5 |
Mutated nucleotides are underlined
Concentration of ligand at half maximum normalized GFP fluorescence
GFPmax – GFPmin
Hill coefficient, a measure of cooperativity within the MphR biosensor. Values >1 indicate positive cooperativity.