(A) Illustrative representation of Barnes Maze and experimental protocol. On days 5, 9, and 13 they received an injection of capsaicin (10 mg/kg, i.p.) before trials. Control (n = 9) and AgrpTrpv1 mice (n = 19) were tested. (B-C) Latency and distance traveled to reach the escape hole in the learning phase during trials across days. (D) Tracking data from a control animal representing one trial per day during the learning phase. (E-F) Latency and distance traveled to reach the escape hole upon Agrp neuron activation. (G-H) Latency and distance traveled to reach the escape hole three days after the injection of capsaicin, to probe the long-lasting effects of Agrp neuron activation. In B, C, E, F, G, and H symbols represent mean ± SEM. Statistically significant P values are provided in the panels.