Table 2. Comparison of the clinical, immunophenotypic, and genetic characteristics of 48 de novo pediatric B-ALL patients at diagnosis with respect to JL1 expression status.
| Variables | JL1 negative (N = 12) | JL1 positive (N = 36) | P |
|---|---|---|---|
| Sex (M : F) | 6 : 6 | 18 : 18 | 0.999§ |
| Age (month) | 84.8 ± 64.7 | 72.3 ± 52.6 | 0.506† |
| WBC ( × 109/L) | 77.15 ± 169.10 | 47.99 ± 95.99 | 0.461† |
| Hb ( × 10 g/L) | 9.03 ± 3.01 | 9.38 ± 10.49 | 0.910† |
| PLT ( × 109/L) | 56,000 ± 48,090 | 70,670 ± 60,310 | 0.449† |
| Blast in PB (%) | 65.00 ± 30.93 | 54.36 ± 32.72 | 0.328† |
| Blast in BM (%) | 83.85 ± 17.00 | 84.02 ± 15.11 | 0.975† |
| Days from Dx to 1st CR | 60.5 ± 47.3 | 45.8 ± 36.3 | 0.268† |
| Hematologic CR achievement (%) | 12/12 (100.0%) | 35/36 (97.2%) | 0.560§ |
| Relapse after 1st CR (%) | 3/12 (25.0%) | 2/35 (5.7%) | 0.097§ |
| JL1 expression* (%) | 5.60 (1.00–19.00) | 65.23 (20.00–99.00) | < 0.001‡ |
| CD45 expression (%) | 76.09 ± 29.20 | 82.97 ± 16.63 | 0.316† |
| CD34 expression (%) | 38.34 ± 42.69 | 51.17 ± 40.34 | 0.352† |
| TdT expression (%) | 53.51 ± 28.69 | 59.09 ± 29.06 | 0.566† |
| CD13 expression (%) | 13.04 ± 17.62 | 9.13 ± 15.73 | 0.474† |
| CD33 expression (%) | 8.98 ± 16.68 | 10.55 ± 22.27 | 0.825† |
| CD10 expression* (%) | 67.40 (0.00–94.00) | 93.70 (0.00–99.00) | 0.022‡ |
| CD19 expression (%) | 75.83 ± 24.79 | 82.75 ± 17.31 | 0.289† |
| CD20 expression (%) | 11.55 ± 23.49 | 10.12 ± 16.15 | 0.815† |
| cCD22 expression (%) | 31.72 ± 28.52 | 35.75 ± 29.16 | 0.679† |
| cIgM expression* (%) | 2.35 (0.00–7.00) | 7.00 (0.00–85.00) | 0.003‡ |
| sIgM expression (%) | 3.08 ± 4.15 | 4.94 ± 5.47 | 0.285† |
| RGA from PCR (%) | 4/12 (33.3%) | 15/36 (41.7%) | 0.739§ |
| PCR results (N of patients) | ETV6-RUNX1 (2) | ETV6-RUNX1 (4) | NA |
| BCR-ABL1 (1) | BCR-ABL1 (2) | ||
| FUS-ERG (1) | FUS-ERG (1) | ||
| KMT2A-AFF1 (1) | KMT2A-AFF1 (1) | ||
| Normal (7) | TCF3-PBX1 (6) | ||
| KMT2A-MLLT1 (2) | |||
| Normal (20) | |||
| Karyotype results (N of patients) | Hyperdiploidy (3) | add(9)(p21) (1) | NA |
| t(8;9)(q24.3;p13) (1) | t(9;22)(q34;q11.2) (2) complex (5) | ||
| t(16;21)(p11.2;q22) (1) | del(9p) (2) | ||
| t(9;22)(q34;q11.2) (1) complex (1) | +10 (1) | ||
| t(4;11)(q21;q23) (1) | t(16;21)(p11.2;q22) (1) hyperdiploidy (6) | ||
| Normal (4) | t(4;11)(q21;q23) (1) | ||
| t(11;19)(q23;p13.3) (2) | |||
| t(2;16)(p11.2;p11.2) (1) | |||
| t(1;19)(q23;p13.3) (6) | |||
| Normal (8) |
*These variables did not show normal distribution in the Kolmogorov-Smirnov test; thus, the median values and ranges are presented. All other continuous variables are presented as mean±SD.
P values were obtained from the Student t-test†, Mann-Whitney U test‡ and chi-square/Fisher's exact test§.
Abbreviations: M, male; F, female; WBC, white blood cells; PLT, platelets; PB, peripheral blood; BM, bone marrow; Dx, diagnosis; CR, complete remission; RGA, recurrent genetic abnormalities included in the 2016 WHO classification; NA, not applicable; CD, cluster of differentiations; TdT, terminal deoxynucleotidyl transferase; c, cytoplasmic; s, surface; t, translocation; add, addition; del, deletion.