Table 1. Analytical performance of the GC-MS assay for three phytosterols.
| Within-run imprecision (%) | Between-run imprecision (%) | ME (%) | Recovery (%) | PE (%) | LLOD (μmol/L) | LLOQ (μmol/L) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Low | High | Low | High | Low | High | Low | High | Low | High | |||
| Cholestanol | 2.13 | 0.82 | 8.64 | 4.92 | 150.8 | 118.7 | 90.0 | 80.2 | 135.8 | 95.2 | 0.3 | 6.8 |
| Sitosterol | 4.37 | 2.57 | 7.32 | 9.06 | 119.0 | 107.4 | 94.9 | 83.5 | 112.9 | 89.7 | 0.2 | 12.1 |
| Campesterol | 1.64 | 1.16 | 10.94 | 10.23 | 114.8 | 103.3 | 95.5 | 82.2 | 109.6 | 84.9 | 0.3 | 7.5 |
Abbreviations: ME, matrix effect; PE, process efficiency; LLOD, lower limit of detection; LLOQ, lower limit of quantification; GC-MS, gas chromatography-mass spectrometry.