Size-exclusion chromatograms of glutenin of HI 977 wheat variety executed under different buffer conditions a 2 M urea, b 3 M urea, c 4 M urea, d 5 M urea, e 6 M urea, f 3 M urea, 1% DTT, and g 3 M urea, 1% SDS. All the buffers were maintained at a pH of 5.5