Table 1.

Primer sets used for PCR

Target	Forward sequences (5' to 3')	Reverse sequences (5' to 3')
CDHR2	AAACCGGTCGCCACCATGGCCCAGCTATGGCTGTCC	AAATCGATTCACAGGTCCGTGGTGTCCAG
myr-AKT	AACCGGTCACCATGGGGAGCAGCAAGAGCAAGCCCAAGATGAGCGACGTGGCTATTGTG	AAATCGATTCACTTATCGTCGTCATCCTTGTAATCGGCCGTGCCGCTGGC
CDHR2 (for RT-PCR†)	ATCATAGGATTGGGAGTGGC	GTCTTTGTTGGGGAGGTTCA
β-Actin (for RT-PCR†)	CCAGCACAATGAAGATCAAG	GAAAGGGTGTAACGCAACTAA
Cox2 (for RT-PCR†)	GAAACTCTGGCTAGACAGCGTAA	AAACCGTAGATGCTCAGGGACT
Methylated CDHR2 promoter (for MSP‡)	ATAGAGAAAAGAGTTTAGGGGTACG	AAACAAAACACAAATAAAAACCGA
Unmethylated CDHR2 promoter (for MSP‡)	TAGAGAAAAGAGTTTAGGGGTATGG	AAACAAAACACAAATAAAAACCAAA

^†RT-PCR, reverse transcription polymerase chain reaction.

^‡MSP, methylation-specific PCR.