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Genetically modified human myogenic cells secrete neurotrophic factors (NTFs). Levels of NTFs were measured using ELISA kits 24 and 72 h after the hMPCs were transduced with lentiviruses containing a gene for expressing either (A) BDNF, (B) GDNF, (C) VEGF, or (D) IGF-1. The levels of NTFs secreted from hMPCs harboring the GFP gene were measured as control values. The data are presented as mean ± SEM, ∗P < 0.05, ∗∗P < 0.01, one-tailed t-test.
