Figure 2.

CHS alters the oligomeric state and secondary structure of VDAC^R. (A) SEC of VDAC^R (blue) and VDAC^R + CHS (red) are shown, with the calculated R_s values displayed above each peak. (B) Averaged CD spectra of VDAC^R (blue) and VDAC^R + CHS (red) with SDs of three measurements are displayed as error bars. For secondary structure deconvolution, see Table 1. (C) A microscale thermophoresis (MST) isotherm of 10 nM fluorescently labeled VDAC^R + CHS titrated against unlabeled VDAC^R + CHS is shown. The K_d of the change in behavior is displayed on the plot. (D) c(s) absorbance and interference distributions of VDAC^R at 0.98 mg/mL are shown. Peaks corresponding to free detergent micelles and the VDAC^R monomer are indicated on the plot. (E) The c(s, f/f₀) interference distribution of VDAC^R + CHS at 0.99 mg/mL used to resolve the three VDAC species present in solution is shown. Peaks corresponding to the free detergent micelles, VDAC dimer, trimer, and tetramer, and a peak of unknown composition are indicated on the plot. (F) Absorbance and interference c(s) distributions of VDAC^R+CHS at 0.99 mg/mL are shown. Peaks corresponding to free detergent micelles and VDAC dimers, trimers, and tetramers and a peak of unknown composition are labeled on the plot. The c(s, f/f₀) distribution in (E) was reduced into a 2D c(s, ^∗) distribution and was used as prior probabilities to resolve the three VDAC species in the c(s) fitting procedure displayed in (F). Peaks corresponding to free detergent micelles and VDAC dimer, trimer, tetramer and a peak of unknown composition are labeled on the plot. In all AUC distributions, detergent peaks were assigned using detergent-only control samples (data not shown). This figure is available in color online. To see this figure in color, go online.