Table 2.

Parameters of VDAC-Detergent Complexes and Detergent Micelles Determined from Sedimentation Velocity Experiments

	dn/dcpa	δD (g/g)b	$\stackrel{-}{\mathit{v}}$ (mL/g)c	S20, watera	Rs (nm)d	MPD (kDa)e,f	MP (kDa)e,f	MD (kDa)e,f
VDACR monomer	0.48 ± 0.01	1.99 ± 0.04	0.78 ± 0.02	4.8 ± 0.05	4.2 ± 0.3	107 ± 7	36 ± 3	71 ± 5
VDACR + CHS dimer	0.43 ± 0.03	1.7 ± 0.1	0.78 ± 0.07	5.3 ± 0.03	5.9 ± 0.3	170 ± 30	60 ± 10	100 ± 20
VDACR + CHS trimer	0.37 ± 0.02	1.32 ± 0.07	0.77 ± 0.06	6.4 ± 0.07	6.9 ± 0.4	220 ± 20	100 ± 10	120 ± 20
VDACR + CHS tetramer	0.42 ± 0.02	1.15 ± 0.07	0.77 ± 0.06	7.3 ± 0.06	7.7 ± 0.4	290 ± 30	130 ± 20	150 ± 20
DM micelles	–	–	–	2.4 ± 0.02	2.8 ± 0.2	–	–	39 ± 3
DM + CHS micelles	–	–	–	3.0 ± 0.1	3.36 ± 0.02	–	–	59 ± 2

^adn/dc_p and ⁴S_{20, water} were determined from AUC c(s) distributions (Fig. S2; Table S1).

^bδ_D was determined using Eq. 3.

^c$\stackrel{-}{\mathit{v}}$ of the protein-detergent complex was calculated from the protein (${\stackrel{-}{\mathit{v}}}_P$) and detergent (${\stackrel{-}{\mathit{v}}}_D$) using Eq. 2.

^dR_s was determined by SEC elution volume relative to elution volumes of proteins with known R_s values for VDAC-detergent complexes and by dynamic light scattering for detergent micelles.

^eM_PD, M_p, and M_D values were calculated using Eqs. 1, 5, and 6, respectively, for VDAC-detergent complexes. M_D for pure detergent micelles was calculated using Eq. 1.

^fThe mass of VDAC^R monomer from sequence is 30.3 kDa.