Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 27;10:280. doi: 10.3389/fmicb.2019.00280

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Conte, Mende, Grainge and Colloms.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Genetic screen for genes involved in S. oneidensis colony color. A mini-ISY100 insertion library was generated and plated on LB/Kan to screen for mutants that had lost the red-brown coloration. (A) Close-up of kanamycin resistant S. oneidensis colonies obtained after conjugation with MFDpir/pISY100mini. Heterogeneity in colony size might be due to fitness differences caused by transposon insertions. Alternatively, small colonies might reflect late (post-plating) transposition events. A mutant with pale colony color is circled in red. (B) Pellets obtained after centrifuging 2.8 ml LB liquid cultures (30°C, overnight) showing the difference in color between the wild-type S. oneidensis (left) and a pale mutant (right).