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. 2019 Feb 27;10:280. doi: 10.3389/fmicb.2019.00280

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Copyright © 2019 Conte, Mende, Grainge and Colloms.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Delivery of a chromosomal “landing pad.” (A) Map of the pISY100mini-LP vector, designed to deliver a “landing pad” for ΦC31 integrase-mediated cassette exchange. The same color scheme as in Figure 1 was used for the plasmid backbone. Two ΦC31 attB sites (light-brown) and the rpsL gene (E. coli 30S ribosomal subunit protein S12 – light-orange) conferring streptomycin sensitivity to an otherwise streptomycin resistant E. coli rpsL mutant strain, were added in the mini-ISY100 transposon sequence. (B) Mini-ISY100-LP integrated on the chromosome and genetic cassette exchange attB/attP recombination. The two attB sites inserted in the landing pad can be used to integrate a gene cassette (dark gray) flanked by two attP sites (pink) via ΦC31 integrase-mediated recombination.