Table 2.
Delivery of the mini-ISY100 transposon in E. coli via chemical transformation and electroporation.
| pISY100mini | pUC71K (control) | |||
|---|---|---|---|---|
| Strain | KanR colonies/reactiona | Transposition frequencyb | Transformation frequencyb | Mini-ISY100 activityc |
| Chemical transformation | ||||
| DH5α | 17.5 ± 3.54 | 1.29 × 10-6± 2.95 × 10-7 | 1.40 × 10-3± 2.71 × 10-4 | 9.21 × 10-4± 2.76 × 10-4 |
| TOP10 | 117 ± 28.9 | 3.57 × 10-6 ± 9.47 × 10-7 | 7.32 × 10-3 ± 7.43 × 10-4 | 4.88 × 10-4 ± 1.39 × 10-4 |
| Electroporation | ||||
| DH5α | 1730 ± 297 | 3.95 × 10-6 ± 1.28 × 10-6 | 5.49 × 10-2 ± 6.45 × 10-3 | 7.19 × 10-5 ± 2.50 × 10-5 |
| TOP10 | 2280 ± 254 | 1.10 × 10-5 ± 2.14 × 10-6 | 7.03 × 10-2 ± 1.72 × 10-2 | 1.56 × 10-4 ± 4.89 × 10-5 |
aNumber of KanR colonies obtained from a single reaction. bNumber of colonies on LB/Kan divided by the number of colonies on LB. cTransposition frequency divided by transformation frequency.