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. 2019 Feb 27;10:280. doi: 10.3389/fmicb.2019.00280

Table 3.

mini-ISY100 transposon activity in E. coli after conjugation.

Strain KanR colonies/reactiona Transposition frequencyb Mini-ISY100 activityc
DH5α 1.05 × 105 ± 2.12 × 104 1.54 × 10-2 ± 8.25 × 10-3 4.20 × 10-1 ± 3.70 × 10-1
TOP10 3.50 × 105 ± 1.71 × 105 1.75 × 10-3 ± 9.71 × 10-4 4.78 × 10-2 ± 4.26 × 10-2

aNumber of KanR colonies obtained from a single reaction. bNumber of colonies on LB/Kan divided by the number of colonies on LB. cTransposition frequency divided by frequency of conjugation using E. coli π3 as recipient (3.66 × 10-2 ± 2.55 × 10-2).