Fig. 5.

The 5-HT and catecholaminergic systems are in close proximity of the CR cells. Coronal cryosections of E18 (a–c, e, and F) and E20 (d, i) rat brains immunostained for TH (red, a–d) or 5-HT (red, e–h) and reelin (green), counterstained with DAPI (blue). b, c Enlargements of the boxed area in a showing the TH fibers in close proximity to the reelin-positive CR cells. d Overview of the relation of TH innervation within the mPFC at E20. Coronal cryosections of an E18 rat brain immunostained for 5-HT (red, e–h, i) and reelin (green), counterstained with DAPI (blue). f Enlargement of the boxed area in e showing the 5-HT fibers in close proximity to the reelin-positive Cajal-Retzius (CR) cells. g Enlargement of the boxed area in f showing the 5-HT fibers in close proximity to the reelin-positive CR cells. h Enlargement of the septal region in e showing the 5-HT fibers in close proximity to the reelin-positive CR cells. i E20 cryosection stained for TH (green), 5-HT (light blue) and reelin (red) and counterstained with DAPI (blue). Inset shows the close proximity of the TH and 5-HT fibers with the CR cells. j Confocal image of the marginal zone showing TH (green) and 5-HT fibers (purple) in close proximity to reelin-positive CR cells (red). Bar in a and e, 300 μm; i, 30 μm; j, 20 μm