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Metformin protects against hypoxia-induced apoptosis in H9C2 cells. (A) TUNEL staining of apoptotic cells subjected to normoxia (N) or hypoxia (H) in the presence or absence of 5 mM metformin (M). (B) Quantification of TUNEL-positive cells from (A). Scale bar is 100 μm. Data represents the mean ± SEM from at least three independent experiments. Statistical analysis was carried out by one-way ANOVA: ∗∗∗P < 0.001 between indicated conditions.
