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. 2019 Mar 5;9:3599. doi: 10.1038/s41598-019-40383-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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F-ara-EdU labeling patterns of the SCN in Arabidopsis primary roots. (a) Outline of the experimental strategy testing whether asymmetric chromosomal segregation (ACS) occurs during QC cell division. SCN, blue outline; QC, green outline. (b) Theoretical illustration of symmetric or ACS upon QC cell division in F-ara-EdU-supplemented media. (c) Representative confocal image of F-ara-EdU labeling of the SCN in 4-DAP seedling roots before transfer (0 DAT) and after 1–3 DAT from 1/2 MS media to F-ara-EdU-supplemented media. Red fluorescence indicates incorporation of F-ara-EdU into newly synthesized DNA during cell division. The QC cells are outlined with dashed lines. DAP, days after planting; DAT, days after transfer; Scale bars, 20 μm.