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. 2019 Mar 5;9:3599. doi: 10.1038/s41598-019-40383-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of cell division rates of various stem cell types in seedlings at 4 and 11 DAP. The graph shows the cell division rates in the QC (left), columella initial (middle), and vascular initial (right) cells. Entry into the S-phase of the cell cycle was monitored in the RAM of primary roots of 4- (black bars) and 11-DAP (red bars) seedlings at 0.5, 1, 2, and 3 DAT from 1/2 MS media to F-ara-EdU-supplemented media. Cells entering the S-phase were determined by red fluorescence of Alexa 555 dye conjugated with F-ara-EdU, which is incorporated into newly synthesized DNA during cell division. Data represent means ± SD (n = 15) from three independent trials. Statistical analysis was performed using two-tailed Student’s t-test (**p < 0.01; ***p < 0.001). DAP, days after planting; DAT, days after transfer.