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. 2019 Mar 5;9:3565. doi: 10.1038/s41598-019-39080-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wound contraction and pigmentation, during the early (c–f) and late (g–i) healing phase. (a) Fish with three punch biopsy wounds. (b) Stereoscope picture of intact skin with the epidermal layer (e), pigment cells (p) and collagenous tissue (c). (c1–f1) Photographs of the wounds during the early healing phase. Pigmented bodies at the wound margins (double arrow). (c2–f2) Stereoscope pictures (40×) of the wound surface. (c3–f3) Stereoscope pictures (16×) of horizontally cut wounds showing the wound bed (wb). From 3 dpw and onward it is also possible to see the epidermal layer covering the entire wounded surface. (g1–i1) Photographs of the contracting wounds during the late healing phase. (g1–i1) Stereoscope pictures (40×) of the wound surface. (g3–i3) Stereoscope pictures (16×) of horizontally cut wounds showing the epidermal layer, pigment cells and collagenous tissue. Photographs N = 12, stereoscope pictures N = 6. Columns represent dpw and rows different orientation and magnification of the wound.