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. 2019 Mar 5;10:1053. doi: 10.1038/s41467-019-09005-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Simultaneous disruptions for 6–8 genes using GTR-CRISPR. a Graphic representation and results of the one-promoter-GTR-CRISPR system for 6–8 genes. b Graphic representation and results of one-fusion-promoter- or two-promoter-GTR-CRISPR system for 6 genes. c Graphic representation and results of simultaneous 8-gene disruptions by the two-promoter-GTR-CRISPR system. The data of bar charts represent mean averages of each gene or overall disruption efficiencies. Each black dot represents the gene disruption efficiency of 10 colonies from each biological replicate, and the error bars indicate the standard deviations of all (n ≥ 3) biological replicates. The statistical analyses are performed using unpaired t Test. Source data are provided as a Source Data file