Fig. 5.

Lighting GTR-CRISPR with un-optimized guide RNAs. a Graphic representations of the L-Histidine synthesis pathway. PRPP phosphoribosyl pyrophosphate, AICAR 5-aminoimidazole-4-carboxamide ribonucleotide. b Graphic representations and result of the Lighting GTR-CRISPR systems for six HIS gene disruptions. The data of bar charts represent mean averages of each gene or overall disruption efficiencies. Each black dot represents the gene disruption efficiency of 10 colonies from each biological replicate, and the error bars indicate the standard deviation of all (n = 6) biological replicates. Source data are provided as a Source Data file