Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 5;9:3450. doi: 10.1038/s41598-019-40107-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Volcano plots and most significant pathways of gene-level differential expression analysis results. Volcano plots in panels a and b represent Wald test contrast results from time-point interval comparisons between diet and control groups. Panels c and d depict results from diet group only analysis. Differentially expressed genes (DEGs) with statistically significant P values (false discovery rate, FDR < 0.05) (y-axis); blue indicates down-regulation and red indicates up-regulation. Magnitude of expression change is depicted on the x-axis with log2FoldChange. Panel e shows pathways associated with the 231 down-regulated DEGs from Time*Group interaction after the weight loss period (PRE-MID). No significant up-regulated pathways were found from the 24 up-regulated DEGs of the Time*Group interaction after the same period. Panel f depicts significant up-regulated pathways associated with 82 DEGs (panel d) affected by the whole weight cycling period in the diet group (PRE-POST) – although these genes were affected only by the weight regain period, not by weight loss. No significant down-regulated pathways were observed from the 5 down-regulated DEGs after the weight cycling period (PRE-POST). In panel g, lipid metabolism-related normalized gene expression levels and color key are represented as standard deviation (SD) change from reference Z-score. Baseline Z-score values (PRE) calculated from both diet and control group were pooled together and set as a reference level to which each individual group/timepoint level were compared; blue indicates decrease and red indicates increase in expression level compared to the reference value. a = Cholesterol synthesis, b = Energy metabolism regulation, c = Fatty acid oxidation, d = Fatty acid synthesis, e = Fatty acid mobilization and transport, f = HDL associated genes, and g = mono-, di-, triglyceride synthesis.