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. 2019 Mar 5;9:3585. doi: 10.1038/s41598-019-40329-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NOX expression in GCs in vitro. (A) RT-PCR identified NOX4/5 and DUOX1/2 in cultured GCs after isolation (d0) and on culture day 3 (pooled GCs from 2–5 individuals). Controls including RNA (-RT) and H₂O instead of cDNA (H₂O) were negative. The displayed figure was cropped from original gel images, which are part of the supplementary data. (B) NOX4 protein was detected in cultured GCs by immunoblotting (anti-NOX4 antibody from ProSci). Controls without primary antibody were negative.