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Knockout of uPAR by CRISPR/Cas9 system. (A) The map of lentiCRISPRv2 vector. (B) The locations and sequences of two sgRNAs of uPAR. (C) The protein expression levels of uPAR were examined by Western blot, and vinculin was used as loading control. The genomic DNA of cells was amplified and sequenced by the designed primers. The sequencing comparison and original data of HCT8/T (D) and KBV200 (E) cells are shown.
