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. 2019 Feb 27;10:304. doi: 10.3389/fimmu.2019.00304

Figure 1.

Figure 1

Dab2 is highly expressed in colonic lamina propria CD103−CD11b+ DCs and downregulated during murine colitis. (A,B) Flow cytometric analysis of DAB2 expression in small intestinal (SI) and colonic (CO) mucosal DCs of healthy WT C57BL/6J mice; **p < 0.01 and ***p < 0.005 when compared to other; ####p < 0.001 when comparing the indicated groups DC subsets from the same tissue. (C,D) Flow cytometric analysis of DAB2 expression in the SI and CO DC in Rag2−/− mice after T cell transfer (Colitis) or injection with PBS (Ctrl) (***p < 0.005 and ****p < 0.001 Colitis vs. Ctrl); n.s., not significant. DCs were analyzed by flow cytometry after staining with viability dye eFluor 506, anti-F480, CD11c, CD11b, CD103, and anti-DAB2 mAbs. DC are depicted as live F480−CD11c+ and stratified by CD11b and CD103 expression. Data represent DAB2 expression as mean fluorescence intensity (MFI) of two independent experiments combined (n = 5 mice/experiment). (E) Western blotting analysis of DAB2 expression (96 kDa) in WT (DC2.4WT) and Dab2−/− (clones 1 and 2; DC2.4Dab2−/−) DC2.4 cells after transfection with Dab2 CRISPR-CAS9 and clonal selection.