Figure 10.

DAB2 modulates autophagy and protects dendritic cells from apoptosis. (A) DC2.4^WT and DC2.4^Dab2−/− cells were left untreated or treated either with 100 nM bafilomycin A1 (BAF) or 200 nM rapamycin (RAP) for 6 h and the expression of autophagy markers, p-UKL-1, UKL-1, p62, LC3A/B I-II, was evaluated by Western blotting. (B) Summary of relative changes in LC3A/B-I expression. (C) Summary of relative changes in LC3A/B-II expression. (D) Summary of relative changes in SQSTM1/p62 expression. (E) Cell death in DC2.4^WT and DC2.4^Dab2−/− cells treated with vehicle or 1 μM staurosporine for 3 h was accessed by flow cytometry to identify AnnexinV⁺7-AAD⁻ apoptotic cells (*p < 0.05 Ctrl DC2.4^WT vs. Ctrl DC2.4^Dab2−/− cells; ****p < 0.001 Stau DC2.4^WT vs. Stau DC2.4^Dab2−/− cells). (F) Caspase-3 activation after incubation with 1 μM staurosporine for 1, 2, and 3 h was accessed using Western blot. The samples compared were separated in the same gel. Due to inter-experimental variation in the time-course of caspase 3 activation, two out of three independent experiments performed are depicted.