Figure 2.

Pro-inflammatory effect of Dab2-deficient DCs during DSS colitis. (A) C57BL/6J mice received 3% DSS in their drinking water or regular water for 8 days. On day 2, mice were intraperitoneally injected with 8.5 × 10⁵ DC 2.4 WT or Dab2^−/− cells. Mice were switched to regular water on day 8. Two cohorts of mice were euthanized on days 4 or 9 after DSS treatment. (B) Relative body weight change; n = 8–11 **p < 0.01 and ****p < 0.001 DSS vs. H₂O in mice treated with DC2.4WT and DC2.4^Dab2−/− cells, respectively. (C) Representative H&E staining of the distal colons from each experimental group (day 9). Bar = 200 μm. (D) Colonic gene expression of selected inflammatory mediators in control and DSS-treated mice by qRT-PCR on days 4 and 9 after DSS treatment. n = 5–11 (*p < 0.05, **p < 0.01 and ***p < 0.005 when compared to untreated mice or ^#p < 0.05 and ^##p < 0.01 in pairs indicated by brackets.