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. 2019 Feb 27;10:304. doi: 10.3389/fimmu.2019.00304

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Copyright © 2019 Figliuolo da Paz, Jamwal, Gurney, Midura-Kiela, Harrison, Cox, Wilson, Ghishan and Kiela.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DAB2 is expressed in bone marrow derived dendritic cells from C57L/6J mice and correlates with CD11b expression. Bone-marrow derived dendritic cells (BMDCs) were differentiated from C57BL/6J mice with a combination of 200 ng/mL FLT3L for 9 days and 20 ng/mL GM-CSF for the last 2 days and analyzed by flow cytometry after staining with viability dye eFluor 506, anti-F480, B220, CD11c, CD11b, CD103, and anti-DAB2 mAbs. Conventional DC are depicted as live F480⁻B220⁻CD11c⁺ and stratified by CD11b and CD103 expression. (A) Relative abundance of BMDC phenotypes based on the expression of CD11b (neg, low, or high) and CD103 (neg, pos) (gates 0–5). (B) DAB2 expression in BMDCs is expressed as mean fluorescence intensity (MFI) in each gate. (C) Representative histograms of DAB2 expression in the populations gates as in (A). Data represent mean values of three independent experiments combined (n = 3 mice/experiment ****p < 0.001 when compared to the gates/populations 0, 1, 2, and 4).