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. 2019 Feb 27;10:304. doi: 10.3389/fimmu.2019.00304

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Copyright © 2019 Figliuolo da Paz, Jamwal, Gurney, Midura-Kiela, Harrison, Cox, Wilson, Ghishan and Kiela.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DAB2 expressed in dendritic cells has an unstable protein but a stable transcript. (A,B) BMDCs were incubated with 5 μg/mL cycloheximide (CHX) for up to 30 min and DAB2 expression was evaluated by western blotting. (C) Time course analysis of Dab2 mRNA after treatment with 10 μg/mL actinomycin D or 5 μg/mL CHX (experiment was limited to 3 h to avoid toxicity and cell death). Dab2 and Tbp gene expression was quantified using qRT-PCR. *p < 0.05, **p < 0.01, ****p < 0.001 effect of the treatment when compared to time 0. Data represent mean values from at least two independent experiments combined (n = 3 mice/experiment).