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. 2019 Mar 5;9:3575. doi: 10.1038/s41598-019-40440-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bacterial survival after treatment with lysozyme (25 mg/L) or lysozyme plus colistin (25 mg/L + 0.025 mg/L), in the phoQ knockout mutant (PW3132) from the UW Library²², and its originary wildtype strain (PAO1-UW). Incubations were performed as explained in materials and methods, and the survival percentage was calculated with regards to the initial inoculum. Each column represents the mean value of at least three independent assays for each strain, whereas the error bar represents the standard deviation (SD). *Statistically significant differences between the two treatments: P < 0.05 in the Student’s t test. The differences among bacteria treated with lysozyme and controls (bacteria incubated in the assay buffer without protein) were statistically significant in all the cases, with a slight growth in the control tubes (bacterial survival > 100%, P < 0.05, data not shown).