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. 2019 Mar 5;9:3595. doi: 10.1038/s41598-019-40054-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative analysis of [¹⁸F]TFAHA PET/CT/(MRI) and immunohistochemical (IHC) staining in U87-MG glioma and in the contralateral hippocampus. The left column: (top) coronal T2-weighted MR image; (middle) corresponding coronal PET/CT/(MRI) fusion image; (bottom) corresponding coronal PET/CT image. Macroscopic (top row) and microscopic images (x200) of axial brain sections stained with H&E and by IHC to visualize the levels of expression of HDACs 4, 5, 9, and acetylation of histones H2AK5ac, H2BK5ac, H3K9ac, and H4K8ac in U87-MG tumor (middle row) and in contralateral hippocampal CA2 (bottom row). PET/CT images are color-coded to standard uptake values (SUV).