Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 5;9:3503. doi: 10.1038/s41598-019-39762-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Sequence dependent errors. (a) Comparison of consensus₁₀ error at each base using different polymerases in the barcoding PCR step. Mean ± SD is shown (n = 3). (b) Spearman’s correlation coefficient between error frequencies by base position and different polymerases using different polymerases in barcoding PCR. All correlations are significant (p < 0.05). (c) Base composition of the reference strand in each amplicon. (d) Mean consensus₁₀ error in each amplicon by nucleotide change relative to the reference sequence. For example, consErrorG of 0.01% reflects that 0.01% of bases were changed to a G from any of the other bases. The observed pattern is congruent with expected polymerase-induced errors based on the base composition of each amplicon. consErrorD denotes deleted bases. HF, High Fidelity; SF, SuperFi.