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. 2019 Mar 5;9:3503. doi: 10.1038/s41598-019-39762-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of SiMSen-Seq sensitivity. Seraseq ctDNA Mutation Mix v2 reference DNA containing known variant alleles in PIK3CA, KIT and BRAF at allele frequencies of 0.125%, 0.0625% and 0.03125% were sequenced with SiMSen-Seq. Arrows indicate the position of the expected variants. The reading frame in the BRAF amplicon indicates codon V600. Consensus₁₀ errors are all non-reference bases present in ≥90% of reads in barcode families with at least 10 reads.