Fig. 2.

Stoichiometry limits the detection of acetylated peptides. a Acetylation is biased to detection on abundant proteins. Protein copy number estimates are from⁴². b The fraction of acetylated lysines detected on any given protein is correlated with protein abundance. The scatterplot shows the % lysines acetylated and copy numbers of 2488 acetylated proteins containing 6753 acetylation sites. The Pearson’s correlation (r), and P-value (P) of correlation are shown. c The number of peptides and acetylated peptides (Ac peptides) detected in deep proteome measurements from this study and¹³. d The distribution of peptide copy numbers from a deep proteome measurement and acetylated peptide copy numbers calculated from the peptide stoichiometry and protein copy number. e The distribution of acetylated peptide copy numbers for acetylated peptides that were detected without prior antibody enrichment. Source data are provided as a Source Data file