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. 2019 Mar 5;9:3611. doi: 10.1038/s41598-019-40453-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of exosomes isolated by electron microscopy and western blot. (A) Image of exosomes isolated at 59000 X in a Tecnai 12 transmission electron microscope with Mega-View III digital camera (Philips, Eindhoven, The Netherlands). An image acquired at 135000 X is shown in the upper right part. (B) Distribution by vesicle size of the isolated exosomes detected by electron microscopy. More than 100 images were recorded and the vesicles were analyzed in each one with the MIP4 Advanced software. (C) SDS-PAGE and western blotting of three different exosome samples isolated from plasma of control subjects. Anti-CD9 was used as primary antibody.