Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 5;9:3449. doi: 10.1038/s41598-019-40251-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CRISPR/Cas9-induced mutations at the zebrafish asip1 locus. (A) Scheme of the asip1 gene showing the target site mutation (black arrowhead). Coding exons are represented as white boxes and 5′ UTR and 3′UTR are shown as black boxes. (B) Sequence of induced deletions in asip1 locus. The first line shows the wild-type sequence. Black arrowhead labels the protospacer-adjacent motif (PAM). Next lines show different induced mutations. Italic lower case letters represent inserted new sequence. The number of deleted (−) and inserted (+) bases are marked on the right side of each sequence. Selected mutations are labeled by white arrowheads. (C) Predicted amino acid sequence encoded for asip1 loci. The first line shows the wild type protein, and following lines show the potential protein sequence of each selected mutation. Grey boxes show the wild type sequence. Asterisk represents the stop codon.