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. 2019 Mar 5;9:3454. doi: 10.1038/s41598-019-40042-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo differentiation of human cremaster muscle-derived myogenic precursor cells. (A) Outline of the experimental design. (B–H) Immunofluorescence of histological sections from the control TA group (B,D,G) and the experimental TA group (C,F,H), respectively. (B,C) Histological analysis of the grafted cell survival through human LAMIN A/C detection by immunofluorescence (green). (D–F) Detection of human LAMIN A/C (green) and PAX7 (red) expressing satellite cells localized in their niche by immunofluorescence (arrows, E). (G,H) Detection of human LAMIN A/C (green) and human DYSTROPHIN (red) positive fibres. Nuclei were counterstained with Hoechst 33258 (blue). Scale bars, 100 μm.