Figure 3.

Changes of Tumor-Infiltrating Immune Cells and Cytokines in Response to Therapy

(A) Post-treatment, KPC-RFP/Luc tumor-bearing mice from intervention groups (n = 3) were randomly sacrificed (day 22). Tumor tissues were harvested, and single-cell suspensions were stained and analyzed by flow cytometry. (B) Immunofluorescence microscopy shows upregulation of IFN-γ in tumor, when treated with ppp dsRNA LCP-AEAA. Scale bar, 100 μm. DAPI stains blue for nuclei, and IFN-γ stains red. (C) Tumor tissues were harvested, and total RNA was isolated followed by cDNA synthesis. The resultant cDNA samples were utilized for gene expression analysis using qPCR. The statistical analyses were performed by comparison with the control PBS group. All data show mean ± SEM (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.