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. 2019 Feb 1;27(3):542–558. doi: 10.1016/j.ymthe.2019.01.016

Figure 4.

Figure 4

miR-183-5p Targets the 3′ UTR of Erbin

(A) A schematic diagram showed wild-type and mutant of miR-183-5p binding sites in the 3′ UTR of Erbin. (B) The Dual-Luciferase Reporter Assay System was used in U937 cells. Luciferase activity was measured at 48 h after transfection. (C) The biotinylated miR-183-5p or its mutant (miR-183-mut) was transfected into U937 cells. qRT-PCR was applied to quantify the RNA levels of Erbin and GAPDH. Scatterplot showed the relative ratios of the input of IP. **p < 0.01.