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. 2019 Feb 1;27(3):542–558. doi: 10.1016/j.ymthe.2019.01.016

Figure 5.

Figure 5

miR-183-5p Negatively Regulates Erbin in HL-60 and U937 Cells

(A) miR-183-5p negatively regulates the expression of Erbin at the mRNA and protein levels. HL-60 cells were transfected with miR-183 inhibitor, and U937 cells were transfected with miR-330 mimic. qRT-PCR and western blotting analysis were used to determine the expression of Erbin at the mRNA and protein levels, respectively. HL-60 and U937 were transfected with miR-183 inhibitor, miR-183 mimic, or empty control. (B) CCK-8 assay was used to measure cell proliferation. (C) Flow cytometry was applied to determine cell-cycle distribution. (D) Annexin V assay was used to detect cell apoptosis. *p < 0.05 versus control.