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. 2019 Feb 1;27(3):542–558. doi: 10.1016/j.ymthe.2019.01.016

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Rescue Experiment with miR-183-5p/Erbin in HL-60 and U937 Cells

The expression of Erbin at the mRNA (A) and protein (B) levels was measured by qRT-PCR and western blotting analysis, respectively. The viability (C, HL-60; D, U937) and cell-cycle distribution (E, HL-60; F, U937) were measured by CCK-8 assay and flow cytometry, respectively. These experiments were conducted after transfection with control vector, miR-183 mimic or miR-183 inhibitor, Lv-Erbin or Lv-shErbin, and miR-183 mimic+Lv-Erbin or miR-183 inhibitor+Lv-shErbin. *p < 0.05 versus control.