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. 2019 Feb 1;116(5):807–817. doi: 10.1016/j.bpj.2019.01.026

Figure 2.

Figure 2

Characterization of ANS binding to CcdB at pH 4 and 7. (a) and (b) show fluorescence spectra and fluorescence intensity at 475 nm, respectively, for CcdB, CcdA, and MBP (maltose-binding protein) in the presence of ANS. CcdB binds ANS only at pH 4 (cyan) and not at pH 7 (pink). The CcdA peptide (residues 46–72) is reported to be largely unstructured yet shows comparable ANS binding (brown) to CcdB at pH 4. The CcdA-CcdB complex (green) at pH 4 also binds to ANS, suggesting CcdB retains exposed hydrophobic patches even in complex with CcdA. Also shown as a positive control are data for the molten globule (MG) of MBP (blue), which is known to bind ANS. To see this figure in color, go online.