Fig. 2.
Effect of undulating topography on keratinocyte proliferation, differentiation and morphology. (A, B, D) Representative images (maximum intensity projections) of cultured keratinocytes labelled for the markers indicated on (A, D) patterned and (B) flat PDMS for the length of time shown. Scale bars: 100 μm. (C, F) Quantification of percentage of involucrin positive cells (% Inv+) (C), Ki67+ cells (F) cell density (F). The total % involucrin-positive cells per topography regardless of location is shown in the middle panel of (C). (E) Quantitation of nucleus volume (nucl. Vol.). *P < 0.05, *** P < 0.001, ****P < 0.0001. (n = 3, 9 images (each corresponding in area to 0.40 mm2) per group were analysed).