Immunofluorescence labelling of keratinocytes on patterned substrates 24 h post seeding. Representative images (maximum intensity projections) showing expression of F-actin (A), E-cadherin (B), Desmoglein 3 (C) and MAL (D) with DAPI counterstain (blue). Scale bars: 100 μm. (E) Quantitation of immunofluorescence images. **P < 0.01, ***P < 0.001, ****P < 0.0001. n = 3 independent experiments, 9 images (each corresponding in area to 0.40 mm2) per experiment. one-way ANOVA for E-cadherin, F-actin, Desmoglein 3 and paired t-test for MAL.