Biomechanical properties of the cell body and cell-cell junctions of keratinocytes cultured on flat substrates. (A) Representative images of immunolabled keratinocytes following culture in low or high calcium FAD, or high calcium FAD containing ROCKi. Scale bars: 100 μm. (B) Representative images of height and Young’s modulus map. (C) Quantitation of mean Young’s modulus of cell-cell borders and cell body. **P < 0.01, ***P < 0.001, ****P < 0.0001. (n = 3, 9 random (50 × 50 μm) areas per group, 20 measurements per area).