Fig. 6.

Effect of culturing keratinocytes on topographies with different slopes. (A) SEM images of uncoated patterned PDMS with steep (A) and shallower (B) slopes. (C-F) Representative images (maximum intensity projections) of keratinocytes immunolabelled for β1 integrin (red) with DAPI counterstain (blue). Cells were cultured for the number of days shown in the presence or absence of ROCKi. (C, E) steep, (D, F) shallow slopped topographies. Scale bars: 200 μm (A, B), 100 μm (C–F). (n = 3, 9 images per group (each corresponding in area to 0.40 mm²) were analysed).