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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: J Immunol. 2019 Jan 30;202(6):1680–1685. doi: 10.4049/jimmunol.1800898

Figure 2. In vivo IL27 treatment fail to protect mice from lethal EAE without Tregs.

Figure 2.

(A) EAE induction and Treg depletion were performed as described in Figure 1. A miniosmotic pump containing rIL-27 was subcutaneously implanted 7 days post induction: sham (n = 5), IL-27 pump (n = 5), and DTx with IL-27 pump (n = 6). Mice were daily monitored for clinical disease and survival. (B) Total number of CNS infiltrating CD4 T cells was enumerated 14 days post induction. (C) B6 wild type or B6 Foxp3DTR mice induced for EAE were treated with DTx with or without IL-27 pump (n = 5~10) and sacrificed 14 days post EAE induction. The CNS tissues were harvested and the indicated cytokine gene expression was measured by qPCR. Each symbol represents individually tested animal. Data are mean ± SD of three independent experiments. * p< 0.05; ** p< 0.01; *** p< 0.001.