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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: J Immunol. 2019 Jan 30;202(6):1680–1685. doi: 10.4049/jimmunol.1800898

Figure 3. IL27 signaling in Treg is necessary for protection of EAE.

Figure 3.

(A and B) Groups of Foxp3DTR mice were induced for EAE. Treg depletion, IL-27 pump implantation, and Treg transfer were performed as described in Figures 1 and 2. WT or Il27ra−/− Tregs were used. Mice were daily monitored for clinical diseases and survival. (C) CNS infiltrating CD4+ T cell number was calculated 14 days post induction. (D) In vivo BrdU incorporation was determined as described in the Materials and Methods. (E) qPCR analysis was performed to assess TNFα, IFNγ, IL17, Rorc, and T-bet gene expression in the CNS. Each symbol represents individually tested animal. Data are mean ± SD of three independent experiments. * p< 0.05; ** p< 0.01; *** p< 0.001.