Fig 5. IL27 induces IL-10 expression in vitro but not in vivo.
(A) Naive CD4 T cells were stimulated in conditions promoting Th1, Th2, Th17, and iTreg differentiation. IL-27 was added during the stimulation. Il10 mRNA expression at the end of 3-day culture was determined by qPCR analysis. (B and C) Groups of B6 or Foxp3DTR mice were induced for EAE and treated with DTx alone or together with IL-27 pump as described in Figure 2. Upon sacrifice at 14 days post induction, (B) CNS and dLN cells were isolated and ex vivo restimulated with MOG peptide for 48h. IL-10 and IFNγ secretion in the culture supernatant was determined by ELISA. (C) In addition, serum levels of IFNγ, TNFα, IL-17 and IL-10 were measured using a CBA assay. Each symbol represents individually tested animal. Experiments were repeated at least twice with similar results. * p< 0.05; ** p< 0.01; *** p< 0.001; **** p<0.0001.