Figure 5. IL-10R blockade does not affect Bcl-2 or CD25 expression on T cells in the brain during chronic infection.
(A-C) Effector T cell populations isolated from the brain were analyzed by flow cytometry following α-IL-10R blockade. (A) Representative histograms showing Bcl-2 expression on effector CD4+ and CD8+ T cells isolated from the brains of chronically infected mice treated with control or α-IL-10R blocking antibody. (B) The MFI of Bcl-2 on effector T cells isolated from the brains of control or α-IL-10R-treated mice (n=5–6 per group, data is representative of three independent experiments and analyzed using Student’s t-test). (C) The frequency of CD25+ effector T cells isolated from the brains of chronically infected mice after control or α-IL-10R treatment (n= 6 per group, data is representative of two independent experiments and analyzed using Student’s t-test). * denotes p<0.05, ** denotes p<0.01, and *** denotes p<0.001 for all panels.