Table 2.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pSM174 | pRS316 (CEN, URA3) | [48] |
| pSM2673 | pRS316::PPGK1-10His-3HA-zmpste24H335A | [7] |
| pSM2676 | pRS316::PPGK1-10His-3HA-zmpste24P248L | [7] |
| pSM2677 | pRS316::PPGK1-10His-3HA-ZMPSTE24 | [7] |
| pSM2982 | pRS316::PPGK1-10His-3HA-zmpste24L94P | [7] |
| pSM3094 | pRS316::PPGK1-10His-3HA-STE24 | [13] |
| pSM3186 | pRS316::PPGK1-10His-3HA-zmpste24Y399C | [13] |
| pSM3173 | pRS304::NatMX-PPRC1-10His-3myc-LMNA(431–664) | [13] |
| pSM3177 | pRS304::NatMX-PPRC1-10His-3myc-LMNA(431–664, L647R) | [13] |
| pSM3178 | pRS304::NatMX-PPRC1-10His-3myc-LMNA(431–646) | [13] |
| pSM3377 | pRS316::PPGK1-10His-3HA-zmpste24L94A | This study |
| pSM3378 | pRS316::PPGK1-10His-3HA-zmpste24P248A | This study |
| pSM3379 | pRS316::PPGK1-10His-3HA-zmpste24Y399A | This study |
| pSM3175 | pRS316::PPGK1-10His-3HA-Zmpste24(mouse) | This study |
| pSM3202 | pRS316::PPGK1-10His-3HA-ZMPSTE24 (human, codon-optimized) | This study |
| pSM3371 | pRS315::PPRC1-10His-3myc-LMNA(431–664) | This study |
| pSM3391 | pRS313::PPRC1-10His-3myc-LMNA(431–664) | This study |
| pSM3392 | pRS313::PPRC1-10His-3myc-LMNA(431–664, L647R) | This study |
| pSM3428 | pRS304::NatMX-PPGK1-10His-3HA-ZMPSTE24 (human, codon optimized) | This study |
| pSM3512 | pRS315::PPRC1-10His-3myc-LMNA(431–664, C661S) | This study |
| pSM3513 | pRS315::PPRC1-10His-3myc-LMNA(431–664, L647R) | This study |